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mutant substrate proteins  (Sino Biological)


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    Structured Review

    Sino Biological mutant substrate proteins
    Mutant Substrate Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+substrate+proteins/PKA+Substrate/pm28114269-737-2-17
    Average 90 stars, based on 4 article reviews
    mutant substrate proteins - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis.
    Article Snippet: .. WT or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer I (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ -32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. .. To recover the phosphorylated GST-tagged protein, Pierce Glutathione Magnetic Beads (Millipore) were added into reactions and the bound proteins were washed and eluted according to the vendor’s instructions.

    Article Title: The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer.
    Article Snippet: .. Wild-type or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer II (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ-32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. ..

    Incubation:

    Article Title: A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis.
    Article Snippet: .. WT or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer I (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ -32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. .. To recover the phosphorylated GST-tagged protein, Pierce Glutathione Magnetic Beads (Millipore) were added into reactions and the bound proteins were washed and eluted according to the vendor’s instructions.

    Article Title: The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer.
    Article Snippet: .. Wild-type or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer II (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ-32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. ..

    In Vitro:

    Article Title: A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis.
    Article Snippet: .. WT or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer I (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ -32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. .. To recover the phosphorylated GST-tagged protein, Pierce Glutathione Magnetic Beads (Millipore) were added into reactions and the bound proteins were washed and eluted according to the vendor’s instructions.

    Article Title: The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer.
    Article Snippet: .. Wild-type or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer II (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ-32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. ..

    Kinase Assay:

    Article Title: A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis.
    Article Snippet: .. WT or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer I (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ -32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. .. To recover the phosphorylated GST-tagged protein, Pierce Glutathione Magnetic Beads (Millipore) were added into reactions and the bound proteins were washed and eluted according to the vendor’s instructions.

    Article Title: The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer.
    Article Snippet: .. Wild-type or mutant substrate proteins were incubated with 50 μl of in vitro kinase assay buffer II (SignalChem) containing 100 μM ATP (cold reaction) or 10 μCi [γ-32P]ATP and the indicated protein kinase for 1 h at 30 ◦C. ..



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    90
    Sino Biological mutant substrate proteins
    Mutant Substrate Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+substrate+proteins/PKA+Substrate/pm28114269-737-2-17
    Average 90 stars, based on 1 article reviews
    mutant substrate proteins - by Bioz Stars, 2026-10
    90/100 stars
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